Journal: Cell Reports Medicine

Article Title: Macrophage-mimetic photothermal nanotherapeutics regulate mitochondrial homeostasis and inflammatory cascades in lung ischemia-reperfusion injury

doi: 10.1016/j.xcrm.2026.102768

Figure Lengend Snippet: Preparation and characterization of Rg3@PACVs (A–C) Characterization of mPDA, Rg3@mPDA, and Rg3@PACVs using transmission electron microscopy (TEM) and dynamic light scattering (DLS) for hydrodynamic size distribution and zeta potential analysis (scale bars, 100 nm). (D) Elemental mapping of carbon (C), nitrogen (N), oxygen (O), phosphorus (P), and sulfur (S) in Rg3@PACVs (scale bars, 100 nm). (E) Fourier transform infrared spectroscopy (FTIR) spectra of mPDA, Rg3, Mac-CVs, and Rg3@PACVs. (F) The expression of macrophage-membrane markers including Toll-like receptor 2 (TLR2), integrin subunit alpha M (CD11b), C-X-C motif chemokine receptor 4 (CXCR4), receptor for advanced glycation end-products (RAGE), interleukin-6 receptor (IL-6R), and tumor necrosis factor receptor 1 (TNFR1) detected by digital western blot analysis. (G) Fluorescence of Dio-labeled Mac-CVs and Dil-labeled Rg3@mPDA after ultrasonic extrusion to form Dio/Dil-labeled Rg3@PACVs (scale bars, 1 μm). (H) Near-infrared (NIR, 808nm) images of Rg3, mPDA, Mac-CVs, and Rg3@PACVs in microcentrifuge tubes. (I and J) Temperature changes of Rg3@PACVs under different laser power intensities (I) and the photothermal effect curves of Rg3@PACVs at various concentrations (J). (K) Photothermal stability of Rg3@PACVs. (L) Size stability of Rg3@PACVs after 1-week storage at 4°C and 37°C.

Article Snippet: CD11b Recombinant Rabbit Monoclonal Antibody , HUABIO , Cat# HA722075; RRID: AB_3096756.

Techniques: Transmission Assay, Electron Microscopy, Zeta Potential Analyzer, Fourier Transform Infrared Spectroscopy, Spectroscopy, Expressing, Membrane, Western Blot, Fluorescence, Labeling

Journal: Cell Reports Medicine

Article Title: Macrophage-mimetic photothermal nanotherapeutics regulate mitochondrial homeostasis and inflammatory cascades in lung ischemia-reperfusion injury

doi: 10.1016/j.xcrm.2026.102768

Figure Lengend Snippet: Rg3@PACVs ameliorate lung injury by suppressing inflammatory responses in rat lung ischemia-reperfusion injury (IRI) (A) Lung injury assessment after tail vein injection of Rg3, PACVs, or Rg3@PACVs with near-infrared (NIR) irradiation during 2-h reperfusion following 1-h pulmonary ischemia. (B) IVIS images of major organs harvested 30 min and 1, 2, 4, 8, 12, and 24 h after intravenous injection. (C–E) Plasma levels of inflammatory cytokines IL-6 (C), IL-1β (D), and TNF-α (E). (F) Total cell counts in bronchoalveolar lavage fluid (BALF). (G and H) Hematoxylin and eosin (H&E) staining of lung tissue (scale bars, 50 μm) and lung injury scores. (I) Wet/dry weight ratio assessing pulmonary edema. (J and K) Immunofluorescence analysis of (J) neutrophil marker CD11b (red); (K) myeloperoxidase (MPO) intensity reflecting neutrophil degranulation (green); scale bars, 50 μm. (L) Zonula occludens-1 (ZO-1) expression (red) indicating alveolar-capillary barrier integrity; scale bars, 50 μm. (M) Apoptosis detection by TUNEL assay. Nuclei counterstained with DAPI (blue). Scale bars: 50 μm. Data are presented as the mean ± SD and analyzed using one-way ANOVA with Tukey’s post hoc test ( ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 vs. Sham; # p < 0.05, ## p < 0.01, ### p < 0.001 vs. IRI; & p < 0.05, && p < 0.01, &&& p < 0.001 vs. IRI+Rg3@PACVs; n = 5 biological replicates).

Article Snippet: CD11b Recombinant Rabbit Monoclonal Antibody , HUABIO , Cat# HA722075; RRID: AB_3096756.

Techniques: Injection, Irradiation, Clinical Proteomics, Staining, Immunofluorescence, Marker, Expressing, TUNEL Assay